Synthetic Gene Design Using Codon Optimization On-Line (COOL).

Author
Yu, Kai · Ang, Kok Siong · Lee, Dong-Yup
Published 2017
Journal Methods in molecular biology (Clifton, N.J.)
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Abstract

Codon optimization has been widely used for designing native or synthetic genes to enhance their expression in heterologous host organisms. We recently developed Codon Optimization On-Line (COOL) which is a web-based tool to provide multi-objective codon optimization functionality for synthetic gene design. COOL provides a simple and flexible interface for customizing codon optimization based on several design parameters such as individual codon usage, codon pairing, and codon adaptation index. User-defined sequences can also be compared against the COOL optimized ones to show the extent by which the user's sequences can be evaluated and further improved. The utility of COOL is demonstrated via a case study where the codon optimized sequence of an invertase enzyme is generated for the enhanced expression in E. coli.

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Details

Title
Synthetic Gene Design Using Codon Optimization On-Line (COOL).
Author
Yu, Kai · Ang, Kok Siong · Lee, Dong-Yup
Published
2017
Year
2017
Journal
Methods in molecular biology (Clifton, N.J.)
Type
Research Article
Language
eng
PMID
27671929
Keywords
Genes, Synthetic · Codon · Genes
This is Version 1 of this record. We added this version on September 30, 2016. This version is based on an original data import from MEDLINE®/PubMed®.