A Guide to Using STITCHER for Overlapping Assembly PCR Applications.

Author
O'Halloran, Damien M
Published 2017
Journal Methods in molecular biology (Clifton, N.J.)
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Abstract

Overlapping PCR is commonly used in many molecular applications that include stitching PCR fragments together, generating fluorescent transcriptional and translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR is also used for genotyping and in detection experiments using techniques such as loop-mediated isothermal amplification (LAMP). STITCHER is a web tool providing a central resource for researchers conducting all types of overlapping assembly PCR experiments with an intuitive interface for automated primer design that's fast, easy to use, and freely available online.

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Details

Title
A Guide to Using STITCHER for Overlapping Assembly PCR Applications.
Author
O'Halloran, Damien M
Published
2017
Year
2017
Journal
Methods in molecular biology (Clifton, N.J.)
Type
Research Article
Language
eng
PMID
27671928
Keywords
Polymerase Chain Reaction
This is Version 1 of this record. We added this version on September 30, 2016. This version is based on an original data import from MEDLINE®/PubMed®.