PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly.

Author
Trubitsyna, Maryia · Liu, Chao-Kuo · Salinas, Alejandro · Elfick, Alistair · French, Christopher E
Published 2017
Journal Methods in molecular biology (Clifton, N.J.)
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Abstract

Joining DNA sequences to create linear and circular constructs is a basic requirement in molecular biology. Here we describe PaperClip, a recently developed method, which enables assembly of multiple DNA sequences in one reaction in a combinatorial manner. In contrast to other homology-based multi-part assembly methods currently available, PaperClip allows assembly of a given set of parts in any order without requiring specific single-use oligonucleotides for each assembly order.

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Details

Title
PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly.
Author
Trubitsyna, Maryia · Liu, Chao-Kuo · Salinas, Alejandro · Elfick, Alistair · French, Christopher E
Published
2017
Year
2017
Journal
Methods in molecular biology (Clifton, N.J.)
Type
Research Article
Language
eng
PMID
27671936
Keywords
Dna
This is Version 1 of this record. We added this version on September 30, 2016. This version is based on an original data import from MEDLINE®/PubMed®.