Rapid Assembly of DNA via Ligase Cycling Reaction (LCR).

Author
Chandran, Sunil
Published 2017
Journal Methods in molecular biology (Clifton, N.J.)
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Abstract

The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the ligase chain reaction (LCR). The LCR method utilizes non-overlapping DNA parts that are ligated together with the guidance of bridging oligos. Using this method, we have successfully assembled up to 20 DNA parts in a single reaction or DNA constructs up to 26 kb in size.

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Details

Title
Rapid Assembly of DNA via Ligase Cycling Reaction (LCR).
Author
Chandran, Sunil
Published
2017
Year
2017
Journal
Methods in molecular biology (Clifton, N.J.)
Type
Research Article
Language
eng
PMID
27671935
Keywords
Dna
This is Version 1 of this record. We added this version on September 30, 2016. This version is based on an original data import from MEDLINE®/PubMed®.