Interleukin-2 as a marker for detecting asymptomatic individuals in areas where Leishmania infantum is endemic.

Author
Ibarra-Meneses, A V · Carrillo, E · Sánchez, C · García-Martínez, J · López Lacomba, D · San Martin, J V · Alves, F · Alvar, J · Moreno, J
Published online 2016-06-03
Journal Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
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Abstract

No field method exists for identifying asymptomatic individuals in areas where Leishmania infantum is endemic. This work reports that, 24 h after stimulating whole blood with soluble Leishmania antigen (SLA), plasma interferon-γ (IFN-γ) and interleukin-2 (IL-2) become significantly elevated in samples from asymptomatic individuals (n=47) compared with those from negative controls (n=50), all of them recruited from a blood bank. When compared with the reference test SLA-lymphoproliferative assay, IL-2 appears as a new, 100% sensitive and specific marker for asymptomatic individuals with a positive cellular response (compared with 100% and 84.78%, respectively, for IFN-γ). Further studies in other transmission areas and in other cohorts of exposed people need to be performed to confirm these results. Once validated, IFN-γ and IL-2 levels in SLA-stimulated whole blood could be reliably used in the field to estimate the prevalence of those asymptomatic individuals with Leishmania-specific cellular immune responses.

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Details

Title
Interleukin-2 as a marker for detecting asymptomatic individuals in areas where Leishmania infantum is endemic.
Author
Ibarra-Meneses, A V · Carrillo, E · Sánchez, C · García-Martínez, J · López Lacomba, D · San Martin, J V · Alves, F · Alvar, J · Moreno, J
Published online
2016-06-03
Published
2016-08
Year
2016
Journal
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
Type
Research Article
Language
eng
PMID
27265372
Keywords
Leishmania · Interleukin-2 · Leishmania infantum
This is Version 1 of this record. We added this version on September 8, 2016. This version is based on an original data import from MEDLINE®/PubMed®.