Branched polyethylene glycol for protein precipitation.

Author
Sim, Siow-Leng · He, Tao · Tscheliessnig, Anne · Mueller, Monika · Tan, Reginald B H · Jungbauer, Alois
Published online 2011-12-28
Journal Biotechnology and bioengineering
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Abstract

The use of linear PEGs for protein precipitation raises the issues of high viscosity and limited selectivity. This paper explores PEG branching as a way to alleviate the first problem, by using 3-arm star as the model branched structure. 3-arm star PEGs of 4,000 to 9,000 Da were synthesized and characterized. The effects of PEG branching were then elucidated by comparing the branched PEG precipitants to linear versions of equivalent molecular weights, in terms of IgG recovery from CHO cell culture supernatant, precipitation selectivity, solubility of different purified proteins, and precipitation kinetics. Two distinct effects were observed: PEG branching reduced dynamic viscosity; secondly, the branched PEGs precipitated less proteins and did so more slowly. Precipitation selectivity was largely unaffected. When the branched PEGs were used at concentrations higher than their linear counterparts to give similar precipitation yields, the dynamic viscosity of the branched PEGs were noticeably lower. Interestingly, the precipitation outcome was found to be a strong function of PEG hydrodynamic radius, regardless of PEG shape and molecular weight. These observations are consistent with steric mechanisms such as volume exclusion and attractive depletion.

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Details

Title
Branched polyethylene glycol for protein precipitation.
Author
Sim, Siow-Leng · He, Tao · Tscheliessnig, Anne · Mueller, Monika · Tan, Reginald B H · Jungbauer, Alois
Published online
2011-12-28
Published
2012-03
Year
2011
Journal
Biotechnology and bioengineering
Type
Research Article ·
Language
eng
PMID
22012585
Keywords*
Chemical Precipitation · Immunoglobulin G · Chemistry · Polyethylene Glycols · Polyethylene · Precipitation

Fields edited by Q-Sensei or Q-Sensei's users are marked with an asterisk (*).
This is Version 3 of this record. Q-Sensei Corp. added this version on June 4, 2012. It is an edited version of the original data import from MEDLINE®/PubMed®. View changes to the previous version or view the complete version history.