A new total antioxidant potential measurements using RP-HPLC assay with fluorescence detection.

Author
Głód, Bronisław K · Piszcz, Paweł · Czajka, Katarzyna · Zarzycki, Paweł K
Published 2011-05
Journal Journal of chromatographic science
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Abstract

In this paper, an improved total antioxidant potential (TAP) estimation using high-performance liquid chromatographic (HPLC) assay with fluorometric detection has been described. The principle of this method is based on the hydroxyl radicals generated in the Fenton-like reaction and subsequently detected using hydroxyterephthalic acid (HTPA), which is a reaction product of hydroxyl radicals and terephthalic acid (TPA), working as a sensing compound. HTPA quantity in the samples was measured by fluorescence detector working at excitation and emission wavelengths equal to 312 and 428 nm, respectively. A number of key experimental conditions including the influence of the reaction (incubation) time on the surface areas of HTPA peaks, concentration of Fe(II) ions as well as the influence of concentration of TPA on the surface area of the chromatographic peak of HTPA were optimized to the characteristic feature of TAP measurements. The elaborated assay has been used to evaluate TAP values of selected low-molecular mass compounds like pyrogallol, tryptamine, and n-alcohols (methanol, ethanol, and n-propanol) as well as chlorogenic and ascorbic acids and benzoic acid derivatives, which are commonly present in the food samples.

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Details

Title
A new total antioxidant potential measurements using RP-HPLC assay with fluorescence detection.
Author
Głód, Bronisław K · Piszcz, Paweł · Czajka, Katarzyna · Zarzycki, Paweł K
Published
2011-05
Year
2011
Journal
Journal of chromatographic science
Type
Research Article
Language
eng
PMID
21549033
Keywords*
Antioxidants · Analysis · Chromatography, High Pressure Liquid · Methods · Chromatography, Reverse-Phase · Spectrometry, Fluorescence · Fluorescence · Measurement

Fields edited by Q-Sensei or Q-Sensei's users are marked with an asterisk (*).
This is Version 3 of this record. Q-Sensei Corp. added this version on January 10, 2012. It is an edited version of the original data import from MEDLINE®/PubMed®. View changes to the previous version or view the complete version history.